suPARnostic® AUTO Flex ELISA Kit – the golden standard for suPAR detection
About suPARnostic® AUTO Flex ELISA Kit
The suPARnostic® AUTO Flex ELISA kit (CE/IVD marked) is a fully quantitative immunoassay designed for accurate and reliable measurement of suPAR (soluble urokinase plasminogen activator receptor) in human plasma. suPAR is a stable biomarker reflecting immune activation and chronic inflammation, making it highly valuable in clinical diagnostics and research.
It can be used in academic and clinical research, including randomized clinical trials measuring suPAR alongside other biomarkers, as well as for stratifying patients by immune activation and disease risk.
Read more about suPAR in patient stratification and research.
It can also be used in longevity and specialized labs, enabling integration into biomarker panels for assessing biological age, inflammation, and immune status, and supporting personalized health assessments and preventive care strategies.
Read more about suPAR in longevity.

How the suPARnostic® AUTO Flex ELISA Kit Works
The assay is based on a double monoclonal antibody sandwich ELISA:
- Samples are mixed with peroxidase-conjugated anti-suPAR detection antibody.
- Incubation occurs in anti-suPAR catching antibody precoated microwells.
- suPAR concentrations are expressed in ng/mL and calibrated against an internal Golden Standard, ensuring comparability across labs and assay lots.
Why Choose the suPARnostic® AUTO Flex ELISA Kit?
- Gold standard in research: More than 80% of peer-reviewed publications on suPAR use this assay, highlighting the necessity for standardized laboratory equipment, and ensuring direct comparability across studies and disease areas.
- Standards create the calibration curve for accurate quantification.
- Clinically approved & patent protected: The only CE/IVD approved ELISA assay validated for clinical decision-making in patient stratification and prognosis.
- Curve control ensures that every run is valid.
- Free ViroGates software automatically converts OD readings into suPAR concentrations (ng/mL).
- Proven clinical impact: suPARnostic® is the most widely used and cited suPAR test in scientific literature.
- Superior assay performance: Comparative studies show higher accuracy and reproducibility than alternative assays (PubMed: 31551522, 35567697). It detects both full-length suPAR (D1D2D3) and the clinically important D2D3 fragment, excludes suPAR/uPA complexes for optimal prognostic value, and delivers reliable, clinically actionable results through stringent lot-to-lot consistency.
Product Details
The suPARnostic® AUTO Flex ELISA kit is CE-IVD certified. It provides fully quantitative results in less than 1½ hours. The kit contains easy-to-use reagents and is flexible with 12×8 strips where up to 91 single samples can be measured on one plate.
- Format: 96-well plate (up to 91 samples per run)
- Assay Time: <1.5 hours
- Sample Type: Plasma (15 µL per replicate)
- Measuring Range: 0.4 – 16.0 ng/mL
- Shelf Life: 1 year from production, store at 2–8 °C
Product contains
- Mixing plate
- Precoated anti-suPAR microtiter plate (12 × 8 break-apart wells)
- Recombinant suPAR standards (calibrated against Golden Standard)
- Curve control
- Peroxidase conjugate
- Dilution buffer
- TMB substrate solution
- Wash buffer (10× concentrate)
- Stop solution
- Sealing tape and accessories
Product Code
Product no. E001: suPARnostic® AUTO Flex ELISA kit for 41-91 tests





