suPARnostic® AUTO Flex ELISA Kit – the golden standard for suPAR detection

About suPARnostic® AUTO Flex ELISA Kit

The suPARnostic® AUTO Flex ELISA kit (CE/IVD marked) is a fully quantitative immunoassay designed for accurate and reliable measurement of suPAR (soluble urokinase plasminogen activator receptor) in human plasma. suPAR is a stable biomarker reflecting immune activation and chronic inflammation, making it highly valuable in clinical diagnostics and research.

It can be used in academic and clinical research, including randomized clinical trials measuring suPAR alongside other biomarkers, as well as for stratifying patients by immune activation and disease risk.

Read more about suPAR in patient stratification and research.

It can also be used in longevity and specialized labs, enabling integration into biomarker panels for assessing biological age, inflammation, and immune status, and supporting personalized health assessments and preventive care strategies.

Read more about suPAR in longevity.

How the suPARnostic® AUTO Flex ELISA Kit Works

The assay is based on a double monoclonal antibody sandwich ELISA:

  • Samples are mixed with peroxidase-conjugated anti-suPAR detection antibody.
  • Incubation occurs in anti-suPAR catching antibody precoated microwells.
  • suPAR concentrations are expressed in ng/mL and calibrated against an internal Golden Standard, ensuring comparability across labs and assay lots.

Why Choose the suPARnostic® AUTO Flex ELISA Kit?

  • Gold standard in research: More than 80% of peer-reviewed publications on suPAR use this assay, highlighting the necessity for standardized laboratory equipment, and ensuring direct comparability across studies and disease areas.
  • Standards create the calibration curve for accurate quantification.
  • Clinically approved & patent protected: The only CE/IVD approved ELISA assay validated for clinical decision-making in patient stratification and prognosis.
  • Curve control ensures that every run is valid.
  • Free ViroGates software automatically converts OD readings into suPAR concentrations (ng/mL).
  • Proven clinical impact: suPARnostic® is the most widely used and cited suPAR test in scientific literature.
  • Superior assay performance: Comparative studies show higher accuracy and reproducibility than alternative assays (PubMed: 31551522, 35567697). It detects both full-length suPAR (D1D2D3) and the clinically important D2D3 fragment, excludes suPAR/uPA complexes for optimal prognostic value, and delivers reliable, clinically actionable results through stringent lot-to-lot consistency.

Product Details

The suPARnostic® AUTO Flex ELISA kit is CE-IVD certified. It provides fully quantitative results in less than 1½ hours. The kit contains easy-to-use reagents and is flexible with 12×8 strips where up to 91 single samples can be measured on one plate.

  • Format: 96-well plate (up to 91 samples per run)
  • Assay Time: <1.5 hours
  • Sample Type: Plasma (15 µL per replicate)
  • Measuring Range: 0.4 – 16.0 ng/mL
  • Shelf Life: 1 year from production, store at 2–8 °C

Product contains

  • Mixing plate
  • Precoated anti-suPAR microtiter plate (12 × 8 break-apart wells)
  • Recombinant suPAR standards (calibrated against Golden Standard)
  • Curve control
  • Peroxidase conjugate
  • Dilution buffer
  • TMB substrate solution
  • Wash buffer (10× concentrate)
  •  Stop solution
  • Sealing tape and accessories

Product Code

Product no. E001: suPARnostic® AUTO Flex ELISA kit for 41-91 tests

Documentation

See technical documents

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FAQs

suPAR is the soluble urokinase plasminogen activator receptor — a circulating form of the receptor (uPAR) that can reflect immune activation and inflammation.

The suPARnostic® AUTO Flex ELISA is an in vitro diagnostic assay used to quantitatively measure soluble urokinase plasminogen activator receptor (suPAR) levels in human plasma. It is intended to aid in the detection and evaluation of inflammatory disorders and immune activation.

The suPARnostic® AUTO Flex ELISA is intended for professional use by trained laboratory technicians in central laboratories. It is typically used for testing samples from patients in Emergency Departments (ED) or Intensive Care Units (ICU). The assay is also used by researchers in clinical and research studies to study the role of inflammation and immune activation in various diseases.

The assay uses EDTA plasma samples. Each replicate requires 15 μL of plasma

The measurable range of the suPARnostic® AUTO Flex ELISA is 0.4 ng/mL to 16.0 ng/mL

The suPARnostic® AUTO Flex ELISA kit should be stored at 2–8°C. Do not freeze. Before use, check the expiry date on the label.
If not stored correctly, the kit’s stability may be affected, and inefficient and misleading results may be obtained.

  • White mixing plate and clear microtiter plate pre-coated with anti-suPAR antibody
  • Five recombinant suPAR standards
  • Curve control
  • Peroxidase conjugate
  • Dilution buffer
  • TMB substrate (3,3’,5,5’-tetramethylbenzidine)
  • Wash buffer (10× concentrated)
  • Stop solution (0.45 M sulfuric acid)
  • Sealing tape
  • Empty plastic bottle for preparation of the conjugate working solution

The assay procedure involves a 1-hour incubation at room temperature (18–26°C), followed by a 20-minute substrate incubation before absorbance is measured.

suPAR values are calculated from absorbance readings at 450 nm. ViroGates provides a free calculation software tool at www.virogates.com to determine suPAR concentrations based on the standard curve.

In healthy individuals aged 18–65 years, median suPAR levels are about 2.2 ng/mL for men and 2.6 ng/mL for women. Patients in emergency departments typically have levels between 3.0 and 6.0 ng/mL, while those with severe disease or organ failure often exhibit double-digit suPAR concentrations.

Population GroupTypical suPAR Level (ng/mL)Notes
Healthy men (18–65 years)2.2 (25–75% range: 1.8–2.9)Median level in healthy blood donors
Healthy women (18–65 years)2.6 (25–75% range: 2.1–3.2)Median level in healthy blood donors
Emergency Department (ED) patients3.0–6.0Reflects typical acute medical presentations
Severely ill patients / organ failureDouble-digit valuesIndicates high immune activation and poor prognosis

The suPARnostic® brand consists of three products:

Quick Triage

A Point of Care Solution

Quick Triage

Turbi-Latex

For Automated Systems

TurbiLatex

ELISA Assay

Clinical and Research

ELISA

Read 1000+ published suPAR studies in leading medical journals

suPAR and inflammation in Nature Medicine medical and scientific Journal
suPAR and inflammation in The New England Journal of Medicine
suPAR and inflammation in Science AAAS Journal
suPAR and inflammation in JAMA medical and scientific journal
suPARnostic® by ViroGates